Scientists at the Eunice Kennedy Shriver National Institute for Child Health and Human Development (NICHD) have developed a method implemented as pulse sequences and software to be used with magnetic resonance imaging (MRI) scanners and systems. This technology is available for licensing and commercial development. The method allows for measuring and mapping features of the bulk or average apparent diffusion coefficient (ADC) of water in tissue – aiding in stroke diagnosis and cancer therapy assessment.
Researchers at the National Cancer Institute developed silicone hydrogel support structures that mimic tissue vasculature (e.g., capillary bed) with high oxygen diffusivity. Photolithographic methods are used to construct mimetic silicone hydrogel pillars that have, for example, a 20:1 height to diameter ratio. Advantageously, these mimetic silicone hydrogels diffuse oxygen from the bottom chamber to the cells cultured on the surface at near physiological rates (60 times that of water).
A variety of techniques have been used to isolate specific cells or cell populations from a histological sample under direct microscopic visualization. Techniques that involve manual or micro-manipulation devices to isolate individual cells based upon visible characteristics and/or immunological staining are labor intensive and can require an extended amount of time.
A Microfluidic Flow-Through Immunoassay for a Simultaneous Detection of Multiple Proteins in a Sub-Microliter Biological Sample
The device consists of a series of channels that are connected via communication ports for sample flow. The channels can be individually loaded with detection reagents via portals at their ends.