Neutralizing Antibodies Against West Nile Virus
West Nile virus (WNV) is a mosquito-borne virus that can cause severe disease affecting the brain and nervous system, especially in older adults and people with weakened immune systems. There is no approved human vaccine or specific antiviral treatment for WNV.
Researchers at NIAID’s Vaccine Research Center (VRC), together with collaborators at Sheba Medical Center and the Israeli Ministry of Health, have identified and characterized seven new fully human monoclonal antibodies that bind to the WNV envelope (E) protein—the main surface protein the virus uses to enter cells. In laboratory studies, these antibodies (AIS-196, AIS-204, AIS-259, AIS-260, AIS-261, AIS-262, and AIS-265) strongly blocked WNV infection, and several also showed protective effects in a mouse model.
The invention includes the antibody sequences and tools needed to produce them, supporting development of full-length antibody therapies or smaller antibody fragments. These antibodies could help prevent WNV disease in people at higher risk or treat infection early, either individually or in combination. Modified versions are also included that may extend how long the antibodies remain active in the body or adjust how they interact with the immune system. The antibodies may also be useful in laboratory tests for WNV diagnosis, surveillance, and research.
- Prevention or treatment antibodies for WNV, especially for people at higher risk of severe disease or after a known exposure
- Fully human antibodies that strongly neutralize virus infection by targeting its key surface E protein
- Flexible formats for different uses, including full-length antibodies or antibody fragments, and the option to use a single antibody or a combination (“cocktail")
- Engineered versions designed to last longer in the body and tune immune functions for safety and performance
- High-quality antibodies for WNV testing and surveillance, supporting laboratory detection, public health monitoring, and research
- Neutralizing antibodies as components of delivery systems for prophylactic or therapeutic applications
- An antibody-based approach for WNV prevention or treatment, given the lack of an approved human vaccine, specific antiviral treatment, or licensed antibody therapy
- Strong virus-neutralizing activity
- Fully human antibodies, which are less likely to cause anti-drug immune responses than non-human or humanized antibodies
- Engineered versions that may last longer in the body and tune immune activity to improve safety and effectiveness
- High-quality antibodies that support WNV prevention or treatment and can also be used in diagnostic tests, public health surveillance, and research