Technology ID
TAB-4860
Advancements in Cytokine Gene Research: Non-Destructive Detection and Isolation through Genetically Modified Mice
E-Numbers
E-039-2020-0
Lead Inventor
Paul (Estate Of), William
Lead IC
NIAID
Co-Inventors
Chen, Xin
Applications
Therapeutics
Research Materials
Diagnostics
Therapeutic Areas
Immunology
Development Stages
Prototype
Research Products
Research Equipment
Plasmids/Vectors
Animal Models
This groundbreaking technology entails the utilization of genetically modified mice, specifically engineered to enable the non-destructive detection and isolation of cells actively transcribing the IL-4 and IL-13 genes. By expressing Amcyan and DsRed-DR fluorescent proteins under the control of these gene loci, researchers gain a powerful tool for studying cytokine gene expression without the need for cell death, offering significant advantages over traditional intracellular staining methods. This innovative approach simplifies experiments, facilitates microscopic observations, and opens new avenues for immunology and cell biology research.
Commercial Applications
This technology has broad applications in immunology and cell biology. It enables real-time studies of immune responses, identification of disease-related cell populations, and investigation of IL-4 and IL-13 roles in various conditions. It also supports drug development by isolating and analyzing cells involved in cytokine signaling pathways. Longitudinal studies are feasible, offering insights into gene expression changes over time. Ultimately, this technology enhances our understanding of cytokine gene regulation and its implications for health and disease.
Competitive Advantages
The utilization of genetically modified mice for non-destructive detection and isolation of cells transcribing IL-4 and IL-13 genes offers several competitive advantages. These mice simplify experimental setups by providing a dual-reporter system, reducing the need for multiple animal models. Moreover, their non-destructive nature preserves cell viability, enabling dynamic, long-term studies, and expanding possibilities for novel experimental manipulations. This technology enhances our understanding of cytokine gene expression in immunology and cell biology research.
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