This technology includes microRNAs for use in cell lines for protein production and potentially future treatments of cancer or diseases related to metabolism. Mmu-miR-466h was identified as a major apoptotic regulator in suspension adapted Chinese Hamster Ovary cells. Mmu-miR-466h was found to have the pro-apoptotic activity by targeting some anti-apoptotic genes for degradation during the exposure of CHO-S cells to the nutrients depleted media. Inhibition of mmu-miR-466h activity resulted in uninhibition of those anti-apoptotic targets and rendered CHO-S more viable with decreased Caspase-3,7 activation during nutrients depleted media incubation. While being pro-apoptotic, mmu-miR466h may also have a tumor suppressor activity in mammalian cells by sensitizing the cancer cells to programmed cell’s death (apoptosis). Mmu-miR-466h is the member of the same microRNA cluster with the pro-apoptotic activity complementary to mmu-miR-466h activity.