This technology includes multiple cells with various combinations of autophagy receptors knocked out. The cell lines include knockouts of the OPTN, NDP52, and TAX1BP1 genes that are involved in cargo selective autophagy. These lines may be used to explore how cell biology debris is catabolized, which may be relevant to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS).
This is a valuable cell series for identifying drugs that function to augment autophagy via specific receptor pathways.
We have made a complete combinatorial autophagy receptor knock out line series, including a penta-KO lines that lacks all five receptors. This offers a unique tool set to probe autophagy mechanism and for drug screening. This includes the following cell lines:
- OPTN KO
- NDP52 KO
- NDP52/0PTN dKO
- NDP52/OPTN/TAX1BP1 tKO