Technology ID
TAB-3474

A Highly Efficient Differentiation Protocol for Placental Cells Derived from Human Pluripotent Stem Cells

E-Numbers
E-169-2019-0
Lead Inventor
Singec, Ilyas (NCATS)
Co-Inventors
Slamecka, Jaroslav (NCATS)
Simeonov, Anton (NCATS)
Applications
Research Materials
Therapeutic Areas
Ophthalmology
Oncology
Infectious Disease
Endocrinology
Dental
Cardiology
Research Products
Research Equipment
Lead IC
NCATS
ICs
NCATS
This technology includes a robust and highly efficient protocol that differentiates human pluripotent stem cells (hPSCs) into the developmental precursor of placental cells, the trophectoderm (TE), under chemically defined conditions. The in vitro generation of TE cells holds great promise for modeling diseases of the placenta, drug screening, and cell-based therapies. Derivation of definitive placental cells from human pluripotent stem cells in culture remains controversial and, so far, placental cells can only be derived directly from primary placental tissue, which largely limits their access and study in the laboratory. Systematic testing of various cell culture conditions and simultaneous manipulation of specific cell signaling pathways has led to the ability to directly induce TE development within 10 days. These cells expressed typical TE markers such as GA T A3, KR T7, and CDX2.
Commercial Applications
The protocol included in this invention will permit increased in vitro use of placental cells for basic research, drug discovery, disease modeling, and cell therapy.

Competitive Advantages
This is the most efficient and most reproducible placental cell differentiation protocol from human pluripotent stem cells.
Licensing Contact:
Vepa, Suryanarayana
sury.vepa@nih.gov
Phone: 301-827-7181