High-Throughput COVID-19 Diagnostic Test that Detects Both Viral and Host Nucleic Acid
NIEHS researchers developed a massively paralleled multiplexed screening method using next generation sequencing (NGS). This method uses sample-specific barcoded indexes that detect both SARS-COV-2 virus and the host’s transcriptional response to infection simultaneously. By matching existing laboratory protocols for PCR-based sample processing, this assay is easily incorporated into existing CLIA-certified facilities. This testing approach provides the capability for testing tens of thousands of patient samples in a large bolus, allowing accurate and fast-turnaround SARS-CoV-2 testing capacity at population scale, and permits monitoring of at-risk individuals.
- Diagnostic test for detecting infectious organisms, including SARS-CoV-2
- Reduction in reagents needed to perform a test, reducing test cost and bottleneck of critical reagents used during nucleic acid amplification
- Simultaneously detect the pathogen and a host’s transcriptional response to infection by the pathogen
- Gene expression information from the donor can be used to predict disease severity
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