Technology ID

Genome Wide DNase I Hypersensitive Sites Detection in Formalin-Fixed Paraffin-Embedded Single Cells

Lead Inventor
Zhao, Keji (NHLBI)
Tang, Qingsong (NHLBI)
Research Materials
Consumer Products
Therapeutic Areas
Development Stages
Development Status
  • Early-stage
  • In vitro data available
Research Products
Research Equipment
Lead IC
A method of detecting DNase I hypersensitive sites ((DHS) in a single cell or very small number of cells, including cells recovered from formalin-fixed paraffin-embedded (FFPE) tissue slides of patient samples. DHS has revealed a large number of potential regulatory elements for transcriptional regulation in various cell types. The application of DNase-Seq techniques to patient samples can elucidate pathophysiological mechanisms of gene function in a variety of diseases as well as provide potentially important diagnostic and prognostic information. Unfortunately, the current DNase-Seq techniques require large number of cells and are applicable only to larger biopsies and surgical specimens. This technique, called Pico-Seq, allows detection when only very small population of cells are available, such as rare primary tumor cells and circulating-tumor-cells, isolated by a variety of methods. Pico-Seq uses conditions capable of restoring the DNase I sensitivity, similar to native/fresh cells, in tissue/cells from slides processed by extremely harsh conditions, such as in FFPE tissues.
Commercial Applications
  • Diagnostic and prognostic kits
  • Research kits
Competitive Advantages
  • Applicable to very small number of cells down to a single cell.
  • Capable of using cells isolated by any of the available methods, including flow cytometry, biopsies, laser capture microdissection, and even cells recovered from formalin-fixed paraffin-embedded tissue slides of patient samples.
Licensing Contact:
Specialist (ALS), Admin. Licensing