Technology ID
TAB-2882
Resolution Enhancement for Line-Scanning Excitation Microscopy
E-Numbers
E-225-2014-0
Lead Inventor
Shroff, Hari (National Institute of Biomedical Imaging and Bioengineering (NIBIB/NIH))
Co-Inventors
York, Andrew (National Institute of Biomedical Imaging and Bioengineering (NIBIB/NIH))
Giannini, John (National Institute of Biomedical Imaging and Bioengineering (NIBIB/NIH))
Kumar, Abhishek (National Institute of Biomedical Imaging and Bioengineering (NIBIB/NIH))
Applications
Software / Apps
Non-Medical Devices
Medical Devices
Diagnostics
Development Stages
Prototype
Development Status
Prototype
Lead IC
NIBIB
The invention describes a method for improving the spatial resolution of optical microscopes that use line-scanning excitation, such as line-scanning confocal microscopes, line-scanning STED microscopes, or line-scanning light-sheet microscopes. Common elements of the invention include: a) an apparatus for exciting and scanning a line-like excitation focus through the sample; b) an optical arrangement on the detection side of the microscope for manipulating the spacing and/or width of the resulting fluorescence emissions; c) integration and optional post-processing of the manipulated fluorescence emissions after capture by an area detector such as a camera. The resolution increase may be performed with no or marginal decrease in temporal resolution relative to the conventional line-scanning microscopes upon which the technique is based.
Commercial Applications
- Fluorescence microscopy
Competitive Advantages
- Improved resolution
- Enhanced acquisition speed relative to other forms of super-resolution microscopy
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