Technology ID

Real-Time RT-PCR Assay for Detection of Noroviruses

Lead Inventor
Vinje, Jan (CDC)
Gregoricus, Nicole (CDC)
Chhabra, Preeti (CDC)
Barclay, Leslie (CDC)
Shirley, Hannah (CDC)
Lee, David (CDC)
Therapeutic Areas
Infectious Disease
Development Status
  • Pre-clinical
  • In vitro data available
Lead IC
A specific and sensitive TaqMan-based real-time (rt) RT-PCR assay has been developed by CDC scientists for detection of noroviruses in clinical and environmental specimens. This assay can be implemented to rapidly detect and distinguish norovirus strains from genogroups I and II, which are responsible for the majority of human infections. Additionally, the assay is multiplexed with an internal extraction control virus (coliphage MS2) to validate the results of the assay. Since the virus cannot be grown in cell culture and enzyme immunoassays lack the necessary sensitivity, this technology is particularly useful.
Commercial Applications
  • Development of norovirus diagnostics
  • Specific rtRT-PCR assay for detecting and distinguishing of the major pathogenic norovirus genogroups (I and II) within clinical and environmental samples
Competitive Advantages
  • This is an internally controlled, multiplexed assay capable of rapid, accurate identification of norovirus genogroups responsible for human illness
  • Superior sensitivity compared with immunoassay detection methods
Licensing Contact:
Mitzelfelt, Jeremiah