Technology ID

Background-Free Fluorescent Nanodiamond Imaging

Bumb, Ambika (NHLBI)
Neuman, Keir (NHLBI)
Development Status
  • Prototype
  • In vitro data available
  • In vivo data available (animal)
Lead IC
Available for licensing and commercial development are intellectual property rights covering a method of imaging a biological specimen (e.g., human tissue) using fluorescent nanodiamonds implanted into the subject of interest, applying a magnetic field to said subject and producing a resultant image by a net juxtaposition of a second acquired image. This process suppresses the background and permits selective imaging of the nanodiamonds in the presence of background fluorescence that exceeds the signal from the nanodiamonds. Another aspect of the invention provides an imaging method in which the resulting image is acquired by applying time-varying magnetic fields using one or more secondary image averaged against the first. The technique relies on imposing a small (~100 Gauss) magnetic field on the sample of interest during optical imaging combined with post-processing of the acquired images to remove the background. This technology can readily be added onto any commercial optical imaging platform to achieve background-free images of the nanodiamonds in a biological specimen.
Commercial Applications
  • In vitro and in vivo optical imaging and diagnostics
  • MRI imaging
Competitive Advantages
  • Improved resolution through composite imagery
  • Background elimination
  • Indefinite tracking due to the exceptional stability of the fluorescent nanodiamonds
  • Wide excitation band (~500-600 nm)
  • Broad-band Near IR emission (600-700 nm)
  • Nanodiamonds are stable in aqueous solution
  • In related technologies we have developed a method to specifically coat and functionalize nanodiamonds for targeting and labeling applications
Licensing Contact:
Shmilovich, Michael