Technology ID

Transgenic Hspa2-Cre Mice for Studying Spermatogenesis and Male Infertility

Lead Inventor
Eddy, Edward (NIEHS)
Research Materials
Development Status
  • In vitro data available
  • In vivo data available (animal)
Lead IC
HSPA2 is a member of the HSP70 family of heat-shock proteins that serve as molecular chaperones. Hspa2-cre expression mimics the spermatogenic cell-specific expression of endogenous HSPA2 within the testis, being first observed in leptotene/zygotene spermatocytes. Expression of the transgene is also detected at restricted sites in the brain, as occurs for endogenous HSPA2.

Researchers at NIEHS developed the first transgenic mouse line that expresses Cre-recombinase under the control of the promoter of the heat shock protein A2 (Hspa2) gene. Expression of the Hspa2-Cre transgene during meiosis in male germ cells makes these mice a useful tool for defining the roles of genes expressed at different times during spermatogenesis or expressed in spermatogenic cells.
Commercial Applications
  • New mouse model to study spermatogenesis and male infertility
  • New mouse model to study meiosis or the roles of heat-shock proteins in general
Competitive Advantages
  • Researchers generated an Hspa2-cre line that expresses cre in spermatocytes to overcome the limitations of other transgenic lines.
Licensing Contact:
Choudhry, Vidita