Technology ID

Composite Probes and Use Thereof in Super Resolution Microscopy

Lead Inventor
Knutson, Jay (NHLBI)
Griffiths, Gary (NHLBI)
Development Status
  • The invention is fully developed
  • Need to build multicolor palette that can be integrated into a commercial microscope
  • May need to make certain protein chimeras and photoinitiators for validation
Lead IC
The technology is in the field of fluorescence microscopy. More specifically, the invention describes and claims the composite probes for super resolution optical techniques using super resolution via transiently activated quenchers (STAQ). The composite probes include a donor moiety and an acceptor moiety joined by a linker. The acceptor moiety, when excited by incident radiation, is excited to a state which, for example, absorbs in the donor emission region, such that the acceptor moiety in its excited state quenches at least a portion of the donor moiety emission. Other transiently activated quenching mechanisms and moieties could accomplish the same task by reducing donor population. Also disclosed are methods for irradiating a selected region of a target material including the composite probe, wherein the composite probe enables improved resolution by point spread function modification.
Commercial Applications
  • Ultrafine imaging for biomolecules, vesicles and organelles, particularly of living biological samples, in biomedical research
  • Potential applications in clinical diagnostics
  • Nanoscopic Lithography - STAQ composites could, in principle, control polymerization of photoresist masks to make feature sizes below 20nm
Competitive Advantages
Improved ultrafine imaging –
  • Imaging objects as small as 10 nm
  • Narrow the point spread function
  • STAQ uses less power, making live cell study practical at theoretically high resolution
Licensing Contact:
Shmilovich, Michael