This technology includes the creation and use of a polyclonal antibody for Neuroligin-4, NLGN4, that was created by injecting a peptide surrounding the pT707 phosphorylation site into rabbits and affinity purifying the resulting serum. Neuroligin-4 is a member of the neuroligin family of cell adhesion proteins. This family has been shown to play a role in the maturation and function of the neuronal synapse and has been implicated in patients with autism and intellectual disability.
The polyclonal antibody against NLGN4 can be potentially used as a biomarker for autism.
This is currently the only antibody to detect the phosphorylation site pT707 of Neuroligin-4. It is also the only antibody that is also specific to Neuroligin 4 in humans.