This invention includes the generation and use of a polyclonal antibody for synapse-associated protein 102 (SAP102) and a polyclonal antibody that binds to mGluR7 when phosphorylated at Serine 862. Peptides of the sites were generated and injected into rabbits to create an immune response. Serum was collected from the rabbits that was then affinity purified. The specificity of the resulting polyclonal antibodies was then determined using biochemical techniques.
They will be used in laboratories as a tool for studying SAP102 and phosphorylated GluR7.
These antibodies are specific to these sites and can be used to detect phosphorylation of the amino acid at this specific site.