Technology Bundle ID

Fluorescent Primer(s) Creation for Nucleic Acid Detection and Amplification

Linked ID
Lead Inventors
Jothikumar Narayanan (CDC)
Vincent Hill (CDC)
Development Stages
Development Status
In vitro data available
CDC researchers have developed technology that consists of a simple and inexpensive technique for creating fluorescent labeled primers for nucleic acid amplification. Fluorescent chemical-labeled probes and primers are extensively used in clinical and research laboratories for rapid, real-time detection and identification of microbes and genetic sequences. During nucleic acid amplification, the "UniFluor" primer is incorporated into newly synthesized double stranded DNA. As a consequence, quenching of the dye's fluorescent signal occurs decreasing the fluorescence of the sample several fold. The decrease in fluorescence can be measured and observed using any commercially available nucleic acid amplification system that measures fluorescence (e.g., real-time PCR/thermocyclers). Because many real-time PCR applications require a multitude of fluorescently labeled primers or probes, the single-labeled primer technique also allows researchers and clinicians to perform their work at lower cost.
Commercial Applications
  • Quantitative detection and/or amplification of specified nucleic acid sequences
  • Efficient fluorescence-labeling of oligonucleotides
  • Pyro-sequencing
  • Basic laboratory research
Competitive Advantages
  • Simple to implement
  • Rapid, real-time detection
  • Used with standard laboratory equipment capable of monitoring fluorescence-intensity shifts
  • Cost-effective
  • Easily adapted for use in kits or arrays

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