Technology Bundle ID

Wild-Type and DNA Polymerase beta Null Mouse Embryotic Fibroblast Cell Lines Harboring a lambda-LIZ Transgene

Research Materials
Medical Devices
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Robert Sobol (NIEHS)
Of great utility in toxicology and DNA repair research are knockout mice with cell lines enabling one to evaluate generations of gene mutations as a direct function of base excision repair. Of particular importance are lambda-LIZ transgenes. Likewise, wild-type and beta-pol null cell lines are equally important. While there exist cell lines carrying the lambda-LIZ transgene, only wild-type cells are currently available. And while wild-type and beta-pol null cell lines exist, none carry the lambda-LIZ transgene.

The present cell line incorporates both of these beneficial properties. These cell lines were created by crossing a transgenic mouse with multiple copies of the lambda-LIZ transgene with a mouse with but a single copy of the DNA polymerase beta. Rebreeding offspring produced cells of both wild type and beta-pol null genotype. The utility of these cells stem from the deficiency in base excision repair as a result of the null mutation in the DNA polymerase beta gene.

Also available for licensing are cell lines created using:
  • Ung KO mice + lambda-LIZ transgene
  • Aag KO mice + lambda-LIZ transgene
  • PMS-2 KO mice + lambda-LIZ transgene
  • Pol-beta/Aag double KO mice + lambda-LIZ transgene
  • Pol-beta/PMS-2 double KO mice + lambda-LIZ transgene
  • Aag/PMS-2 double KO mice + lambda-LIZ transgene

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