Of great utility in toxicology and DNA repair research are knockout mice with cell lines enabling one to evaluate generations of gene mutations as a direct function of base excision repair. Of particular importance are lambda-LIZ transgenes. Likewise, wild-type and beta-pol null cell lines are equally important. While there exist cell lines carrying the lambda-LIZ transgene, only wild-type cells are currently available. And while wild-type and beta-pol null cell lines exist, none carry the lambda-LIZ transgene.
The present cell line incorporates both of these beneficial properties. These cell lines were created by crossing a transgenic mouse with multiple copies of the lambda-LIZ transgene with a mouse with but a single copy of the DNA polymerase beta. Rebreeding offspring produced cells of both wild type and beta-pol null genotype. The utility of these cells stem from the deficiency in base excision repair as a result of the null mutation in the DNA polymerase beta gene.
Also available for licensing are cell lines created using:
- Ung KO mice + lambda-LIZ transgene
- Aag KO mice + lambda-LIZ transgene
- PMS-2 KO mice + lambda-LIZ transgene
- Pol-beta/Aag double KO mice + lambda-LIZ transgene
- Pol-beta/PMS-2 double KO mice + lambda-LIZ transgene
- Aag/PMS-2 double KO mice + lambda-LIZ transgene