This technology includes a HIV-2 plasmid library for use in diagnostic assay development. In December of 2012, the Centers for Disease Control and Prevention (CDC) and the Association of Public Health Laboratories (APHL) proposed a new US laboratory testing algorithm for the diagnosis of HIV infections. This new algorithm recommends additional testing for confirmation of HIV infection using a nucleic acid amplification test (NAAT) in the event of discordant serological test results. This invention is significant because there are few HIV-2 specimens available for use in assay development and optimization. Additionally, there is limited availability of material for use as assay controls and for the monitoring of laboratory quality and operator proficiency.